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qPCR-based mostly quantitative microbiome profiling is relevant also for samples containing a substantial amount of host or non-bacterial DNA, in which bacterial density can't be reliably believed by full DNA produce [five].
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The qPCR primers have a bit decrease but sufficiently similar protection for the domain Microbes in comparison to the primers for NGS. The coverage of the two qPCR and NGS primer sets is highly comparable for that four taxa picked for taxon-precise qPCR (S1B Desk), which provides trusted validation with the explained method Within this examine. For long term advancement with the herein presented method, a qPCR assay employing exactly the same primer pair as for NGS could possibly be optimized and validated.
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